In Silico Design of sgRNAs for CRISPR-Cas9 Targeting of Type 2 Diabetes Genes

This study introduces a computational framework for the design and evaluation of single guide RNAs (sgRNAs) tailored to Type 2 Diabetes Mellitus (T2DM)-associated genetic variants. Using advanced in silico tools, including Benchling for sgRNA optimization and Cas-Offinder for off-target assessment, the framework identifies high-efficiency and high-specificity sgRNAs targeting key genes such as TCF7L2, SLC30A8, KCNJ11, CDKN2A/2B, and IRS1. By systematically analyzing on-target and off-target scores, mismatch tolerance, and bulge effects, the product delivers sgRNA designs that balance precision with performance, offering researchers a reliable foundation for CRISPR-Cas9-based therapeutic development.

The product’s value lies in its ability to streamline the early stages of gene-editing research, reducing trial-and-error in laboratory settings by providing pre-validated sgRNA candidates with benchmarked efficiency and specificity. With projected global diabetes cases surpassing one billion by 2050, this framework supports the urgent need for precision medicine solutions. It empowers biotechnology researchers, healthcare innovators, and pharmaceutical developers to accelerate the transition from genetic insights to actionable therapies, ultimately addressing the root molecular causes of T2DM.

Positioned at the intersection of bioinformatics, genetics, and therapeutic innovation, this product is not only a research outcome but also a tool for advancing CRISPR applications in complex, polygenic diseases. It embodies the principles of accuracy, reliability, and innovation, contributing to next-generation approaches in diabetes management and beyond.

• Innovation/Product Video (Public):
• Category (Public): Star Innovators (Secondary and primary school students)
• Sub-Category (Public): Science & Technology
• Type of Innovation (Public): INNOVATION IDEA (POSTER PRESENTATION) (For innovators who have ideas but not yet any product)
• Country List (Public): Philippines
• Name of Group Leader/ Individual Innovator (Public): Ron Gabriel E. Jogno
• Institution of Group Leader/ Individual Innovator (Public): Tayug National High School
• Name of Members (1): John Rafael C. Datuin
• Institution of Members (1): Tayug National High School
• Name of Members (2): Jedidah Merica S. Pascua
• Institution of Members (2): Tayug National High School
• Name of Members (3): Chris Luen D. Siblas
• Institution of Members (3): Tayug National High School
• Name of Members (4): Shakira Kazhmier R. Pulido
• Institution of Members (4): Tayug National High School